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Abstract: Molecular dynamics simulations with thermodynamic analyses have been employed to determine how DNA adducts derived from the environmental carcinogen benzo[α]pyrene (B[α]P) impact the dynamic structural properties of the damaged DNA duplexes in a sequence- and stereochemistry-dependent manner; we wished to gain insights on the characteristics of the adducts that produce experimentally observed differences in nucleotide excision repair (NER) susceptibilities. About Pro Quest Dissertations & Theses With nearly 4 million records, the Pro Quest Dissertations & Theses (PQDT) Global database is the most comprehensive collection of dissertations and theses in the world.

Our investigation of the major highly mutagenic 10 S ( )-trans-anti-B[α]P-N-d G adduct in the 5'-... It is the database of record for graduate research.

These sequence-dependent dynamic structural variabilities are attributed largely to differences in steric hindrance when nearby guanine amino groups compete with the bulky lesions for accommodation in the minor groove. We also investigated a guanine adduct derived from the highly carcinogenic dibenzo[α, l]pyrene (DB[α,l]P), 14S (-)-trans-anti-DB[α,l]P-N -d G, and obtained detailed structural properties base on NMR data.

duplexes display enhanced dynamic mobility and episodically denatured Watson-Crick hydrogen bonding 5'-flanking the lesion, while the 5'-... duplex is dynamically untwisted concomitant with a larger bend. Additionally, we studied two other B[α]P derived stereoisomers, 10R (-)-trans- and 10R ( )-cis-anti-B[α]P-N-d G adducts, and found that the conformationally different stereoisomeric lesions cause different patterns of minor groove enlargement.

Rosalind Franklin was extremely intelligent and she knew by the age of 15 that she wanted to be a scientist. one of the few institutions at the time that taught physics and chemistry to girls ? When offered the position as an assistant research officer at the British Coal Utilization Research Association (CURA), Franklin gave up her fellowship and took the job.

sequence contexts showed that the B[α]P aromatic moiety is 5'-directed along the modified strand in the B-DNA minor groove in all cases, but with subtle distinctions. Use the link below to access a full citation record of this graduate work:

The results revealed conformational heterogeneity, involving a mix of two conformers: one with the bulky DB[α,l]P moiety 5'-aligned in the minor groove, which is severely distorted, the other with the DB[α,l]P intercalated on the 5'-side of the lesion into the duplex without base displacement.

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url_ver=Z39.88-2004&res_dat=xri:pqdiss&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft_dat=xri:pqdiss:3329842 If your library subscribes to the Pro Quest Dissertations & Theses (PQDT) database, you may be entitled to a free electronic version of this graduate work.




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